Laboratory: Hematology
Test Name:
ADAMTS13 ACTIVITY AND INHIBITOR PROFILE - (P)
Test Code: ADAM
Clinical Information:
Test performed at:  University of Iowa
PRIOR APPROVAL required, except Nephrologists and Hematologists.

Must collect prior to initiating plasma exchange.

Test Indications: It’s essential for  the diagnosis of congenital or acquired TTP (thrombotic thrombocytopenic purpura). Less than 10% ADAMTS-13 activity is highly indicative of TTP in an appropriate clinical setting. TTP is a medical emergency (untreated has nearly 100% mortality and treated in a timely manner has a >90% survival rate), which requires prompt diagnosis, and costly plasma exchange for the patient.

 
Collection Devices:
Specimen Required:
Whole Blood: 4.4 mL
Pediatric Whole Blood: 3.6 mL

Sample must be sent to the lab immediately.

Double spin of plasma required in three (3) separate aliquots. Transfer top two thirds of plasma from collection tube to a new plastic tube.  Re-centrifuge and then remove the top two thirds of plasma and place into a new plastic tube.  Cap and freeze immediately in three (3) separate aliquots

For best results, centrifuged and separated within 60 minutes of collection.
Referral:
Plasma: 2.0 mL
Pediatric Plasma: 1.0 mL



Refer frozen plasma to HSC Central Services on a STAT basis.
Referred Out Location: MORL, Iowa City, IA 
Must be shipped and remain frozen with dry ice.
Freeze plasma immediately at -20°C (-80°C preferred).
One (1) aliquot is retained at HSC Central Services where volume permits. 
 
Requisition:
Reference Values:
See report.
Availability:
Daily
Test must be approved by the Hematopathologist on service, except Nephrologists and Hematologists.
See Also:
More Information:
As per MORL: 
Delivery: Monday to Friday (NO WEEKEND DELIVERIES)
Thawed OR unlabeled samples will be REJECTED for testing.

Specimens cannot be shipped on Friday, holidays, weekends.

Minimum plasma volume: 0.5 mL

Inhibitor studies will be reflexed automatically when Activity is <25%.

Ordering physician to complete Coagulation Patient Information Sheet to accompany the test sample: