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Laboratory:

Clinical Immunology

Test Name:

CYCLIC CITRULLINATED PEPTIDE ANTIBODY (CCP) - (S)

Test Code: CCP

Clinical Information:

Alternate Name(s):
Anti Citrullinated Antibody

Anti Citrulline

Anti-CCP (Cyclic Citrullinated Peptide)

Anti-Citrullinated Ab

Anti-Citrullinated Antibody

Anti-Citrulline Antibody

Anti-Cyclic Citrullinated Peptide (CCP)

CCP (Cyclic Citrullinated Peptide)

Citrullinated Ab

Citrullinated Antibody

Test Indications:

Aids in the evaluation of rheumatoid arthritis. Use of the anti-CCP assay in association with RF assay maximizes the sensitivity/specificity ratio. It must not be forgotten that 15-20% of RAs are positive for RF but negative for anti-CCP and that about half of the RAs negative for RF are anti-CCP positive. Simultaneous positivity for RF and CCP has a positive predictive value of 100%.

Serial monitoring of RA patients who are positive for anti-CCP is not appropriate as these autoantibodies do not correlate with clinical course or with response to drug treatment.

Collection Devices:

Preferred Device:

1 - Serum Separator (SST) Tube(s) - Full Tube Collection

Specimen Required:

Serum: 1.0 mL

Referral:

Serum: 1.0 mL

Shipping & Storage: Separate serum and ship refrigerated, or frozen if greater than 7 days from time of collection.

Testing Laboratory (MB): St. Boniface Hospital Immunology Laboratory

Requisition:

_SH Immunology Autoimmune Laboratory Requisition (R250-10-85)

Reference Values:

Reference Intervals: 0.0 – 4.9 AU/ml
A negative result does not necessarily exclude autoimmune disease. Positive results should not be interpreted in isolation and must be correlated with relevant clinical findings.

Method of Analysis: The IDS CCP assay is based on chemiluminescence technology. Patient serum is incubated with magnetic particles coated with the specific antigen. Following the washing step after the first incubation, an anti-human IgG antibody labelled with acridinium is added, followed by a subsequent incubation step. The magnetic particles are captured using a magnet and a wash step performed to remove any unbound analyte. Trigger reagents are added; the resulting light emitted by the acridinium label is directly proportional to the concentration of anti-CCP IgG in the sample.

Reference: IDS CCP Instructions for Use, IS-AI1201 V09, 17-11-2025

Availability:

Weekdays

TAT: 3-5 Days