MONOCLONAL PROTEIN INVESTIGATION (24 HR URINE) - (U)

Test Code: PEU

Testing is performed at the Health Sciences Centre Immunology Laboratory.
 
Urine proteins are separated in an electric field according to their size, shape, and electric charge.  A pentavalent antisera is applied to the gel forming insoluble complexes with any immunoglobulins present.  A characteristic monoclonal band (M-spike) is often found in the urine of patients with monoclonal gammopathies such as multiple myeloma, primary amyloidosis, and light chain deposition disease. The initial identification of an M-spike or an area of restricted migration is followed by immunofixation to identify the immunoglobulin heavy chain and/or light chain with application of gamma, alpha, mu, kappa, and lambda antisera.
           
When performed at screening, Serum Free Light Chains can replace the 24hr urine protein IFE for all diagnoses except amyloidosis.  However, once a diagnosis of monoclonal gammopathy is made, the 24hr urine protein IFE should be done.
 
Results should be used in conjunction with other laboratory and clinical findings.

_SH Immunology Test Requisition (R250-10-21)

Negative for Monoclonal Protein

Weekdays

Laboratory hours of operation: Monday - Friday 0700 - 1700, excluding holidays. TAT: within 7 days