TISSUE IMMUNOFLUORESCENCE - DIRECT

Test Code: SURG

Alternate Name(s): DIF

HSC:  to request Michel’s Medium contact HSC IMMUNOPATHOLOGY LAB (1-204-787-1513)  (Weekdays:  0800 - 1500 hrs)  External Sites:  fax Pathology Supply Request Form (204-787-4942)
 
Collection in Normal Saline:
Skin biopsy must arrive at HSC Immunopathology lab within 2 hours from collection and before 1500h.
If it will not arrive within 2 hours, see collection in Michel’s Medium.  Place skin biopsy into a sterile jar containing normal saline.
Skin biopsy should not be allowed to dry out.
 
Collection in Michel’s Medium:
Fresh skin biopsy should be placed in Michel’s transport medium ASAP.
Skin biopsy should not be allowed to dry out.
Store in fridge until transported.  Do not freeze.
Do not freeze the transport medium, place on cold pack or ice.
 
Transportation:
1. For sites within Winnipeg, arrange for transportation to HSC Immunopathology lab through established courier service - 1-hour service.
Notify HSC Immunopathology that a skin biopsy is being sent (787-1513).  If procedure occurs at SBH, send to SBH lab and lab will send to HSC.
2. For sites outside of Winnipeg, please contact HSC Immunopathology to arrange transportation of the specimen (1-204-787-1513).  Track unscheduled shipments with a Fax / Fax Back Sheet 170-10-08.

Note: Ensure that specimen will not freeze during transportation to HSC Immunopathology Lab.
 
Internal additional information:  170-10-19, 170-10-18

Pathology Services HSC Laboratory Requisition (80993)

A descriptive report will be issued.

Weekdays

All specimens must be properly labeled with appropriate patient identification. All specimens must be accompanied by a completed Pathology requisition. pertinent information including patient demographics, clinical history, physician’s name and specimen type is required as per DSM Specimen Acceptance Policy 10-50-03.

Biopsies for routine tissue examination are frequently done concurrently with biopsies for DIF studies.
Please ensure biopsies for routine and DIF studies are clearly labelled and submitted appropriately to avoid duplication or omission of the desired investigations required (see Tissue Examination-Routine  section for routine biopsy submission).