Laboratory: Clinical Immunology
Test Name:
Test Code: ILDP
Clinical Information:
May be useful for evaluation of interstitial lung disease in the context of systemic autoimmune rheumatic disease (ie, connective tissue disease).
Collection Devices:
Preferred Device:
1 - Serum Separator (SST) Tube(s) - Full Tube Collection
Specimen Required:
Serum: 1.0 mL

Refrigerated: 14 days
Frozen: 30 days
Serum: 1.0 mL

Shipping & Storage: Separate serum and ship refrigerated, or frozen if greater than 7 days from time of collection.

Testing Laboratory (MB): St. Boniface Hospital Immunology Laboratory
Reference Values:
Reference Intervals: Negative
 A negative result does not necessarily exclude autoimmune disease.  Positive results should not be interpreted in isolation and must be correlated with relevant clinical findings.

Method of Analysis: Interstitial Lung Disease Profile consists of Line Blot Immunoassays for Myositis and Systemic Sclerosis profiles and provides qualitative in vitro determination of human autoantibodies of the immunoglobulin class IgG to the 25 different antigens Mi-2a, Mi-2b, TIF1g, MDA5, NXP2, SAE1, Ku, Scl-70, PM-Scl100, PM-Scl-75, Jo-1, SRP, PL-7, PL-12, EJ, OJ, Ro-52, CENP A, CENP B, RP11, RP155, Fibrillarin, NOR90, Th/To, and PDGFR in serum.  The test kits contain test strips coated with parallel lines of highly purified antigens. In the first reaction step, the immunoblot strips are incubated with diluted patient samples. In the case of positive samples, the specific IgG antibodies (also IgA and IgM) will bind to the corresponding antigenic site. To detect the bound antibodies, a second incubation is carried out using an enzyme-labelled anti-human IgG (enzyme conjugate) catalyzing a color reaction.

1.  EUROLINE Autoimmune Inflammatory Myopathies 16 Ag (IgG) Test Instruction, DL_1530-4G_A_UK_C07.doc, Version 20/03/2018
2. EUROLINE Systemic sclerosis (Nucleoli) profile (IgG) Test Instruction, DL_1532G_A_UK_C06.doc, Version 20/03/2018
Within 1 Week
Ordering restricted to Specialists or by prior approval
See Also:
More Information:
Interstitial Lung Disease Profile will report as a comment:
Interstitial Lung Disease Profile (LIA)
Autoantibodies associated with Interstitial Lung Disease are included in the Myositis and Systemic Sclerosis profiles.  Please see linked results.