Lab Information Manual

Laboratory:

Clinical Immunology

Test Name:

LEUKOCYTE ADHESION DEFICIENCY - (B)

Test Code: LAD

Clinical Information:

CD18, CD15s, CD11a, CD11b, CD11c expression on neutrophils by flow cytometry.
Aids in the diagnosis of Leukocyte Adhesion Deficiency syndrome, type 1 and II, primarily patients < 18 years of age. This flow cytometry assay is intended as a screening test. Screening tests are not 100% sensitive nor specific, and a normal result should not preclude functional assays or molecular sequencing if a patient's clinical presentation suggests that the probability of a diagnosis is high.

Collection Devices:

Preferred Device:	1 - EDTA 4 mL NO GEL Tube(s) - Full Tube Collection EDTA 4 mL NO GEL Tube(s) - Full Tube Collection Manufacturer: Becton Dickinson & Company Catalogue #: 367861
Alternate Device:	1 - Microtainer(s) - EDTA, NO GEL (0.5 mL) to fill line Microtainer(s) - EDTA, NO GEL (0.5 mL) to fill line Manufacturer: Becton Dickinson & Company Catalogue #: 363706

Specimen Required:

Blood: 4.0 mL

Pediatric Blood: 0.5 mL

A CBC and differential must also be performed and must include eosinophil and basophil counts.
Send specimens in original tube. DO NOT ALIQUOT.
Specimens are required to be received in the HSC Immunology Laboratory weekdays and by 4:00 p.m .on Fridays. Draw and package specimen as close to shipping time as possible. It is recommended that specimens arrive within 12 hours of draw to ensure processing within the 24 hour sample stability.
Sample must be shipped at ambient temperature.
Samples arriving on the week-end will be cancelled.

Referral:

Requisition:

_SH Flow Cytometry Laboratory Requisition (R250-10-82)

Reference Values:

Normal (reported as normal or absent expression for each marker). If decreased marker expression is observed, a comment will be included in the report.

Availability:

Weekdays