Lab Information Manual

Laboratory:

Clinical Immunology

Test Name:

ANTI-NUCLEAR ANTIBODY PROFILE - (S)

Test Code: REFLEX FROM HEP 2 [ORDERABLE BY RHEUMATOLOGY ONLY]

Clinical Information:

Test Indications:
Evaluating patients with signs and symptoms of a connective tissue disease in whom the test for antinuclear antibodies is positive. Testing is reflexed based on known pattern/antigen associations.

Testing is not useful in patients without demonstrable anti-nuclear antibodies.

ANA Pattern (≥1:160 Titre)	dsDNA	Profile
NUCLEAR PATTERN
Homogeneous	X	X
Speckled	X	X
Centromere		X
Nucleolar		X
PCNA		X
Cytoplasmic Pattern
Cyto-Speckled		X
Cyto-AMA		X

Visit the International Consensus on Antinuclear Antibody Patterns website for additional information about pattern and disease/antigen associations.

Collection Devices:

Specimen Required:

Alternate Names:
Anti-ENA (Antibodies to Extractable Nuclear Antigens)
Anti-La
Anti-Ribonuclear Protein
Anti-RNP (Anti-Ribonucleoprotein)
Anti-Ro
Anti-Sjogren's Syndrome
Anti-Sm (Smith)
Anti-Smith (SM) Antigen
Anti-SSA (Anti-Sjogren's Syndrome A)
Anti-SSB (Anti-Sjogren's Syndrome B)
Anti-U(1)RNP (Anti-Ribonucleoprotein)
Antibodies to Extractable Nuclear Antigens
Autoantibodies to Jo 1 Antigen
Autoantibodies to Sm (Smith)
Autoantibodies to SSA (Ro)
Autoantibodies to SSB (La)
Autoantibodies to U(1)RNP
CENP B
Centromere Protein B
DFS70
ENA (Extractable Nuclear Antigens), Anti
ENA
Extractable Nuclear Antigens, Anti
Histidyl-tRNA Synthetase Antibodies
Histones
RNP (Anti-RNP)
Ro (Anti-SSA)
Scl 70 Antibodies
Sjogren's Syndrome
Sm (Anti-Sm) (Smith)
Sm (Smith) Autoantibodies
Smith Antigen, Anti
SmRNP Autoantibodies
SSA (Anti-SSA)
SSB (Anti-SSB)
Topoisomerase 1 Antibodies
U(1)RNP (Ribonucleoprotein) Autoantibodies
Autoantibodies to Scl 70 Antigen
Jo 1 Antibodies
LA (Anti-La)
La (Anti-SSB)
Nucleosomes
PCNA
PM-Scl
Ribonucleoprotein (RNP) Autoantibodies
Ribosomal P

Specimen Stability:
Refigerated: 14 days
Frozen: 30 days

Referral:

Testing Laboratory (MB): St. Boniface Hospital Immunology Laboratory

Requisition:

Reference Values:

Reference Intervals: Negative
A negative result does not necessarily exclude autoimmune disease. Positive results should not be interpreted in isolation and must be correlated with relevant clinical findings.

Method of Analysis:
The Line Immunoassay (LIA) provides qualitative in vitro determination of human autoantibodies of the IgG class to 15 different antigens nRNP, Sm, SS-A, Ro-52, SS-B, Scl-70, PM-Scl, Jo-1, CENP B, PCNA, nucleosomes, histones, ribosomal P-protein, AMA-M2 and DFS70 in serum.

References:
EUROIMMUN EUROLINE ANA Profile 3 plus DFS70 (IgG) Test Instruction DL_1590-30G_A_UK_C04.doc Version: 01/02/2019.

Availability:

Weekdays

TAT: Within 4 days

See Also:

More Information:

Interpretation & Assay Interferences:
Positive results will be accompanied by an intensity (Weak, Moderate, Strong)

Autoantibodies Against	Autoimmune disease	Prevalence
nRNP/Sm	MCTD	95%
Sm	SLE	5% - 40%
SS-A	SS or SLE Neonatal lupus erythematosus	40% - 95% or 20% - 60% 95% - 100%
Ro-52	SS or SLE SSc or idiopathic inflammatory myopathy	70% - 90% or 40% - 60% 20% or 20% - 40%
SS-B	SS or SLE Neonatal lupus erythematosus	40% - 95% or 10% - 20% 75%
Scl-70	SSc Diffuse or limited form of SSc	25% - 75% 40% - 65% or 5% - 15%
PM-Scl	SSc including overlap syndrome PM/SSc overlap syndrome SSc (anti-PM-Scl75 positive) SSc (anti-PM-Scl100 positive)	10% - 20% or 5% - 20% 18% 24% - 50% 7%
Jo-1	Myositis (polymyositis/dermatomyositis)	25% - 35%
CENP B	SSc - limited form or SSc - diffuse form PBC	80% - 95% or 8% 10% - 30%
PCNA	SLE	3%
Nucleosomes	SLE	40% - 70%
Histones	Drug-induced SLE SLE or RA	95% - 100% 50% or 15% - 50%
Rib P Protein	SLE	10%
AMA-M2	PBC or other chronic liver diseases SSc	up to 96% or 30% 7% - 25%
DFS70	Atopic dermatitis Rheumatic diseases	4% - 10% 5% - 10%