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Laboratory: Clinical Immunology
Test Name:
Test Code: None
Clinical Information:
Recipient and Donor pairs for kidney transplant purposes only.


HLA Crossmatching is performed to assess compatibility of a donor/recipient pair and the alloimmune risk of the recipient. 


Living Donor:

Virtual Crossmatch – samples are not required.  A virtual crossmatch should be ordered with HLA Typing of a Living Donor and applicable samples for HLA Typing are drawn.


Preliminary crossmatch should be performed on the most suitable living kidney donor within one year of the anticipated transplant date.  Samples are required from both the donor and the recipient.


Final crossmatch should be performed within one week of the transplant date (up to 3 weeks for KPD transplants).  Samples are required from both the donor and the recipient.


Deceased Donor

Final crossmatches are performed urgently as part of the Deceased Donor allocation.  Samples are required from the donor and may be required from the recipient (to be determined on a case by case basis).

Collection Devices:
Specimen Required:
Whole Blood: 34.0 mL

Pediatric: Whole Blood                Volume: Contact TI Lab

The 3 – 8.5mL yellow-top ACD tubes must be collected along with the 8.5 mL SST tube for all recipients.  Donors require 3 – 8.5 mL ACD tubes for all HLA Crossmatches and an 8.5 mL SST tube as well on Final Crossmatches.


Samples must be drawn pre-dialysis.

Ambient: 24 hours

Refrigerated: Do not refrigerate

Frozen: Do not freeze

Samples that arrive within 24 to 48 hours of collection may still be tested, but cell viability may be decreased.


Referral: Whole Blood

Shipping & Storage: Ship at room temperature and should arrive in the lab within 24 hours of collection.

Testing Laboratory (MB): Health Sciences Centre Transplant Immunology Laboratory

Reference Values:
Stat or Routine
See Also:
More Information:
Send specimen to Blood Bank/CBS Transport system for delivery to Transplant Immunology Lab located in the CBS Building.