Laboratory: Clinical Microbiology
Test Name:
Test Code: HPYL
Clinical Information:
Test Indications:
Patients undergoing gastroscopy for dyspepsia, gastritis, active peptic ulcer disease (PUD), a past history of PUD (unless previous cure of H. pylori infection has been documented), low-grade gastric mucosa-associated lymphoid tissue (MALT) lymphoma, a history of endoscopic resection of early gastric cancer (EGC), or currently suspected or history of gastric adenocarcinoma.

Gastric biopsy (minimum of one from antrum and one from body).

Patient Preparation Instructions:
Patient must be off PPI, antibiotics, and bismuth- containing compounds therapy for at least 2 weeks prior to specimen collection.
Collection Devices:
Sterile specimen container with a small amount of saline - Specimens received in other collection devices will not be processed.
Specimen Required: Gastric biopsy (One from antrum and one from body) (Adult and Pediatric)
H. pylori rapid urease tests should be performed on at least two specimens obtained by gastric biopsy, one from the greater curvature of the mid-body and one from the lesser curvature of the antrum Corporal biopsies are especially important in patients with significant gastritis.
Biopsies from separate sites should be submitted separately in saline and promptly delivered to the laboratory. Delays more than one hour will significantly affect results of the test. Alternatively, the biopsies may be placed directly into the urea test broth/agar media available from the microbiology laboratory in some hospital locations.
Antibiotic susceptibility testing is not available for H. pylori.

Transport at ambient temperature. 
Ensure specimens are sent using appropriate packaging in accordance with Transportation of Dangerous Goods Regulation.
Testing Laboratory (MB): Heath Science Center, St. Bonifiace General Hospital, and Westman Lab
Reference Values:
A descriptive report will be provided.
If urease activity is detected from the sample, the sample will be reported as H. pylori detected.
Method of Analysis: Colorimetric pH change of media.
See Also:
More Information:
Specimen Handling:
- Rapid transportation is critical for this specimen type.
- Transport at ambient temperature.
- Specimens should be received by the testing laboratory no more than one hour after collection unless inoculated in appropriate Selective Rapid Urea media.