Lab Information Manual

Laboratory:

Clinical Immunology

Test Name:

ENDOMYSIAL ANTIBODY (IgA) - (S)

Test Code: TTGA

Clinical Information:

Testing is performed at St. Boniface Hospital Immunology Lab using an indirect Immunofluorescence assay (IFA). Testing is performed as a reflex for samples with a positive tTG IgA (initial positive ONLY). Serum samples are initially screened at a 1:10 dilution.

Samples with tTG IgA ≥200 RU/ml will not reflex to AEMA as there is >99% agreement between tTG IgA and AEMA at this threshold.

This test kit is designed for the semiquantitative in vitro determination of human antibodies of immunoglobulin classes IgA against endomysium in patient samples for the diagnosis of gluten-sensitive enteropathy (GSE; young children: coeliac disease, adults: nontropical sprue) and Duhring's dermatitis herpetiformis.

Collection Devices:

Preferred Device:

1 - Serum Separator (SST) Tube(s) - Full Tube Collection

Serum Separator (SST) Tube(s) - Full Tube Collection
Manufacturer: Becton Dickinson

Catalogue #: 367986

Specimen Required:

Blood: 5.0 mL

Serum: 1.0 mL

Referral:

Serum: 1.0 mL

Separate serum and ship refrigerated, or frozen if greater than 7 days from time of collection.

Requisition:

_SH Immunology Autoimmune Laboratory Requisition (R250-10-85)

Reference Values:

AEMA: <1:10 Titre (Negative)

A negative result does not necessarily exclude autoimmune disease. Positive results should not be interpreted in isolation and must be correlated with relevant clinical findings.

Availability:

Weekdays

Laboratory Hours of Operation: Mon-Fri, 0800-1615, excluding holidays. AEMA TAT: within 4 days