Lab Information Manual

Laboratory:

Clinical Immunology

Test Name:

ENDOMYSIAL ANTIBODY (IgA) - (S)

Test Code: TTGA

Clinical Information:

Alternate Name(s):

Celiac Disease

Coeliac Disease

EMA

AEMA

Failure to Thrive

Gluten-sensitive Enteropathy

Test Indications: Aids evaluation of patients for gluten-sensitive enteropathies such as celiac disease and dermatitis herpetiformis (Durhing’s disease).

Endomysial Antibody IgA is performed by reflex testing only from Tissue Transglutaminase IgA.

Collection Devices:

Specimen Required:

Referral:

Testing Laboratory (MB): St. Boniface Hospital Immunology Laboratory

Requisition:

Reference Values:

Reference Intervals: <1:10 Titre (Negative)
A negative result does not necessarily exclude autoimmune disease. Positive results should not be interpreted in isolation and must be correlated with relevant clinical findings.

Note: Levels may become undetectable after a gluten free diet is initiated.

Method of Analysis: Testing is performed using an Indirect Immunofluorescence assay (IFA). This test kit is designed for the semiquantitative in vitro determination of human antibodies of immunoglobulin classes IgA against endomysium in patient samples. Serum samples are initially screened at a 1:10 dilution.

Pediatric TTGA positive samples ≥10.0 AU/ml will automatically reflex to Endomysial antibody testing by immunofluorescence (all instances).

Adult TTGA positive samples in the range 10.0 – 100.0 AU/ml will automatically reflex to Endomysial antibody testing by immunofluorescence (first instance only).

Reference: EUROIMMUN IIFT: Coeliac Disease Screen (LM) EUROPattern Instructions for Use, FC_1914AG_A_UK_C03.doc, Version 07/05/2025

Availability:

Weekdays

Non-orderable, reflex testing only. TAT 5-7days