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Laboratory: Clinical Microbiology
Test Name:
BORDETELLA PERTUSSIS/BORDETELLA PARAPERTUSSIS, MOLECULAR DETECTION, NASOPHARYNGEAL SWAB/NASOPHARYNGEAL ASPIRATE
Test Code: PERT
Clinical Information:
Test Indications:
Clinical suspicion of symptomatic pertussis.  This test SHOULD NOT be performed on asymptomatic contacts of patients with pertussis.  Follow the Manitoba Health contact recommendations for contacts of active pertussis cases.

Recommendations:
A nasopharyngeal swab or aspirate must be obtained for this test.  Dacron or rayon-tipped NP swabs are required.  Cotton and calcium alginate-tipped swabs will be rejected.  A dry swab submitted in a sterile container is preferred.  Throat swabs or nasal swabs are not acceptable.  Only individuals comfortable with obtaining a nasopharyngeal swab or aspirate should obtain the specimen.  Improper collection may result in false negative results.  Detailed instruction on how to obtain a nasopharyngeal swab are located here: https://sharedhealthmb.ca/files/covid-19-sop-swab.pdf

Patient Preparation Instructions:
Accurate patient identification must be made prior to sample collection.
Samples and requisitions must be labeled/completed in accordance with the Shared Health Specimen Acceptance Policy.  Nasopharyngeal swabs may be uncomfortable and patients should be advised of this.
Collection Devices:
Specimen Required: Nasopharyngeal swab or aspirate (Adult and Pediatric) 
Referral:
Requisition:
Reference Values:
Negative for Bordetella pertussis DNA
Negative for Bordetella parapertussis DNA
Availability:
Testing performed Tuesdays and Thursdays
See Also:
More Information:
Specimen Handling:
Submit specimen to laboratory within 2 hours if stored at room temperature.  May be stored up to 48 hours at 2-8ºC.  Ensure specimens are sent using appropriate packaging in accordance with Transportation of Dangerous Goods Regulation.