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Laboratory: Clinical Microbiology
Test Name:
URINE, CULTURE, MIDSTREAM/CATHETER/ILEAL CONDUIT, AEROBIC BACTERIA - (U)
Test Code: MU, CU, UCM, SPU, SPAN, URSW, URVC
Clinical Information:
Test Indications:
A patient with symptoms of dysuria, frequency, urgency and occasionally suprapubic tenderness, in addition, patient may experience flank pain/tenderness with or without fever.

Recommendations:
- Urine specimens obtained with the same collection method within 48 hours of receipt of the first specimen will be rejected as a duplicate specimen.
- Submit urine specimen using a BD Vacutainer Plus C&S preservative tube.
- Collection of midstream urine specimen should be avoided during menses.
- Never collect urine specimen from a bedpan or urinal.
- Please provide clinical information as age, gender, presence of symptoms and specimen type impact specimen work-up.
- Diagnostic Testing Recommendations for Investigation of Urinary Tract Infections (UTI), including pediatric patients:
https://healthproviders.sharedhealthmb.ca/download/706/clinical-biochemistry/28533/diagnostic-testing-recommendations-for-investigation-of-urinary-tract-infections.pdf

Patient Preparation Instructions:
- Obtain early morning specimens whenever possible or urine 4 hours from last micturition to decrease the number of false-negative results.
- Have the patient collect voided urine directly into a sterile leak-proof container, instructing the patient to not halt and restart the urinary stream but preferably move the container into the path of the already voiding urine.
- Female:  hold labia apart then begin voiding.
- Male (uncircumcised):  retract foreskin then begin voiding.
- Male (circumcised):  begin voiding.
-  Catheter urine:  using a needle and syringe, collect urine through the catheter port, after cleansing with alcohol.  Do NOT send urine obtained from a catheter bag.
-  Ileal conduit urine:  Remove the external device.  Cleanse the stoma with 70% alcohol followed by iodine.  Remove the iodine with 70% alcohol.  Insert a double catheter into the cleansed stoma to a depth beyond the fascial level and collect the urine.
Collection Devices:
BD Vacutainer Plus C&S preservative tube (grey top) used in conjunction with the sterile screw-cap collection cup with integrated transfer device.  The BD Vacutainer Plus C&S preservative tube maintains bacteria in urine specimen for 2 days at room temperature allowing for transport time.
Specimen Required:
Urine: 3.0 mL
Pediatric Urine: 3.0 mL

BD Vacutainer Urine Collection Device or Sterile specimen container that holds approx. 100mL
BD Vacutainer:  Minimum 3 mL as indicated by minimum draw line on tube.
Referral:

Transport BD Vacutainer Plus C&S preservative tubes at ambient temperature.  Urine specimen submitted in container with boric acid preservative are stable at room temperature for 48 hours, by which time they must be processed by the lab.
Testing Laboratory (MB): Health Science Center, St. Boniface Hosp., Westman Lab, The Pas Health Complex
Requisition:
Reference Values:
A descriptive report with quantitation will be provided.
Availability:
Daily
See Also:
More Information:
Specimen Handling:
Transport BD Vacutainer Plus C&S preservative tube at ambient temperature.
Transport sterile, leak-proof container (without preservatives) to the lab within 2 hours of collection, otherwise refrigerate for a maximum of 24 hours, by which time they must be received by the lab
Urine greater than 24 hours old not in preservative will not be processed.