Celiac Disease

Coeliac Disease

Tissue Transglutaminase (tTG)

Tissue Transglutaminase Ab IgG

Transglutaminase(tTG)

Gliadin IgG

Test Indications: Aids evaluation of IgA deficient patients for gluten-sensitive enteropathies such as celiac disease and dermatitis herpetiformis (Durhing’s disease). 

Deamidated Gliadin IgG is performed by reflex testing only from Tissue Transglutaminase IgA.  Samples determined to be IgA deficient (≤0.07g/L), and all children ≤3years of age, will automatically reflex to Deamidated Gliadin IgG.  

A decrease in Deamidated Gliadin IgG antibody concentration after initiation of a gluten-free diet may indicate a response to therapy.  IgG antibody titre decreases more slowly than does the IgA antibody count.

Method of Analysis: The IDS Deamidated Gliadin IgG assay is based on chemiluminescence technology. Patient serum is incubated with magnetic particles coated with the specific antigen.  Following the washing step after the first incubation, an anti-human IgG antibody labelled with acridinium is added, followed by a subsequent incubation step. The magnetic particles are captured using a magnet and a wash step performed to remove any unbound analyte. Trigger reagents are added; the resulting light emitted by the acridinium label is directly proportional to the concentration of anti-Deamidated Gliadin IgG in the sample.

Samples determined to be IgA deficient (≤0.07g/L) during TTGA testing, and all children ≤3years of age, will automatically reflex to Deamidated Gliadin IgG. 

Reference: IDS Deamidated Gliadin IgG Instructions for Use, IS-AI1302 V09,17-11-2025