| Laboratory: | Clinical Immunology |
| Test Name: |
DOUBLE STRANDED DNA ANTIBODY (dsDNA) - (S)
Test Code:
DNA
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| Clinical Information: |
Alternate Name(s):
Anti-DNA Anti-ds (Anti-Double Stranded) DNA ANTI-dsDNA ANTIBODY, IgG Antibody to ds-DNA Antibody to Native DNA (n-DNA) DNA Double Stranded (ds-DNA) Antibody DNA, Anti Double Stranded DNA ds-DNA Antibody IgG Anti-DS DNA Antibody IgG Autoantibodies to Double Stranded (ds)DNA Native Anti-DNA Native DNA Test Indications: Secondary screening for systemic lupus erythematosus (SLE) based on ANA results. Also useful for monitoring SLE disease activity. Recommendations: DO NOT order dsDNA as a routine screen with ANA. |
| Collection Devices: |
Preferred Device:
1 - Serum Separator (SST) Tube(s) - Full Tube Collection
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| Specimen Required: |
Serum: 1.0 mL
Specimen Stability: Refrigerated: 14 days Frozen: 30 days |
| Referral: |
Serum: 1.0 mL
Shipping & Storage: Separate serum and ship refrigerated, or frozen if greater than 7 days from time of collection.
Testing Laboratory (MB): St. Boniface Hospital Immunology Laboratory |
| Requisition: | |
| Reference Values: |
Reference Intervals:
0 - 99 IU/ml A negative result does not necessarily exclude autoimmune disease. Positive results should not be interpreted in isolation and must be correlated with relevant clinical findings. Method of Analysis: The ELISA test kit provides a quantitative in vitro assay for human autoantibodies of the IgG class against double-stranded DNA (dsDNA) in serum. |
| Availability: |
Within 3 or 4 days. ELISA tests are batched tests.
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| See Also: | |
| More Information: |
Interpretation & Assay Interferences:
dsDNA is automatically reflexed for Homogeneous and Speckled ANA patterns with titres ≥160. dsDNA is also orderable as a stand-alone test for monitoring SLE disease activity. Increasing antibody levels may be associated with flares while decline or negative results may indicate response to treatment or disease remission. The sensitivity in clinically characterized patients with SLE was 60%. The specificity in a control panel of patients with other autoimmune diseases and health blood donors amounted to 99%. dsDNA reportable range 10 – 800 IU/ml Reference: EUROIMMUN Anti-dsDNA-NcX ELISA (IgG) Test Instructions, EA_1572G_A_UK_C05.doc, Version 22/01/13 |