Lab Information Manual

Laboratory:

Genomics (MOLECULAR DIAGNOSTICS)

Test Name:

Lynch syndrome- MLH1 gene Mennonite variant (c.2141G>A, p.Trp714Ter, W714X) - (B)

Test Code: MD

Clinical Information:

Description: One selected gene variant in MLH1 (variant-c.2141G>A, p.Trp714Ter) is found in some Manitoba Mennonite families.
This testing will not detect variants outside of the regions examined
Ordering restricted to: Medical Genetics

Collection Devices:

Preferred Device:	2 - EDTA 4 mL NO GEL Tube(s) - Full Tube Collection EDTA 4 mL NO GEL Tube(s) - Full Tube Collection Manufacturer: Becton Dickinson & Company Catalogue #: 367861
Alternate Device:	2 - Microtainer(s) - EDTA, NO GEL (0.5 mL) to fill line Microtainer(s) - EDTA, NO GEL (0.5 mL) to fill line Manufacturer: Becton Dickinson & Company Catalogue #: 363706 - Quantity: 2-6 Note that extracted DNA samples can only be accepted from accredited laboratories. Contact Lab Genetic Counsellor at GenomicsLabGC@sharedhealthmb.ca or ph:204-787-4033 with any questions.

Specimen Required:

Whole Blood: 8.0 mL

Pediatric Whole Blood: 1.0 mL

Pediatric: 1.0-3.0 mL
Special Processing: Do NOT centrifuge. Specimen is forwarded to Health Sciences Centre MS551 for analysis.
Specimen Stability:
Ambient: Preferred - store and ship specimens at room temperature
Refrigerated: Accepted
Frozen: Accepted - must remain frozen
Other comments: Specimens sent for Molecular tests are not to be used for other tests (i.e. CBC).

Referral:

Requisition:

_SH Hereditary Cancer Molecular Requisition (R250-10-10)

Reference Values:

Reference Intervals: An interpretive report will be sent.

Availability:

Within 12 Weeks

Routine TAT is 12 weeks. Expedited TAT 4-6 weeks may be requested following laboratory consultation.