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Laboratory:

Clinical Biochemistry (SPECIAL CHEMISTRY)

Test Name:

ALDOSTERONE - (P)

Test Code: ALDO

Clinical Information:

Test Indications: Investigating primary aldosteronism (eg, adrenal adenoma/carcinoma and adrenal cortical hyperplasia) and secondary aldosteronism (eg, renovascular disease, salt depletion, potassium loading, cardiac failure with ascites, pregnancy, Bartter syndrome).

See Hypertension Canada’s 2020 Guidelines for more information.

See F10-50-45D Approved Physicians for Specialist Endocrinology Requisition

Clinical Practice Change - Endocrine Testing and Specialized Endocrinology Requisition Update 15-May-24

Clinical Practice Change General-Endocrinology-Requisition-and-Endocrine-Testing-Update 15-May-24

Collection Devices:

Preferred Device:	1 - EDTA 4 mL NO GEL Tube(s) - Full Tube Collection EDTA 4 mL NO GEL Tube(s) - Full Tube Collection Manufacturer: Becton Dickinson & Company Catalogue #: 367861
Alternate Device:	1 - Microtainer(s) - EDTA, NO GEL (0.5 mL) to fill line Microtainer(s) - EDTA, NO GEL (0.5 mL) to fill line Manufacturer: Becton Dickinson & Company Catalogue #: 363706

Specimen Required:

Plasma: 1.0 mL

Collection Information:

The recommended collection time is 7-10 a.m., after the patient is active (walking, standing, seating) for at least 2 hours. Patient must be seated for 5-15 min prior to collection.

Most aldosterone requests are on the same specimen as renin and therefore requirements for renin collection must be satisfied.

· Collect EDTA plasma. Serum or lithium heparin plasma are not acceptable.

· Do not pre-chill EDTA blood collection tubes nor store tubes on ice, but process blood at room temperature to avoid cryoactivation of prorenin in renin testing.

· Centrifuge tubes in a non-refrigerated centrifuge, separate EDTA-plasma from cells immediately after centrifugation, then aliquot and deep-freeze at -20 °C or below instantly.

Specimen Stability:

Refrigerated: 5 days if only aldosterone is ordered on the specimen (renin is not stable and undergoes cryoactivation)

Frozen: 3 days at -20^oC or at least 1 month at -80°C

Referral:

Plasma: 1.0 mL

Shipping & Storage: Store and ship frozen.

Testing Laboratory (MB): Send specimen to Health Sciences Centre - MS5

Requisition:

_SH General Endocrinology Requisition (R250-10-76)

_SH Specialized Endocrinology Requisition (R250-10-77)

Reference Values:

Reference Intervals:

Age	Gender	Reference Interval
0 – <3y	M/F	83 – 3134 pmol/L
3 - <18y	M/F	144 – 677 pmol/L
≥18y	M/F	Upright: 61 - 978 pmol/L Supine: 32 - 654 pmol/L

Method of Analysis: DiaSorin Chemiluminescence

Availability:

Within 1 Week