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Laboratory: Clinical Biochemistry  (SPECIAL CHEMISTRY)
Test Name:
RENIN DIRECT (MASS, CONCENTRATION) - (P)
Test Code: RENI
Clinical Information:
Test Indications: Investigating primary aldosteronism (eg, adrenal adenoma/carcinoma and adrenal cortical hyperplasia) and secondary aldosteronism (eg, renovascular disease, salt depletion, potassium loading, cardiac failure with ascites, pregnancy, Bartter syndrome).

 

See Hypertension Canada’s 2020 Guidelines for more information.


See F10-50-45D Approved Physicians for Specialist Endocrinology Requisition

Collection Devices:
Specimen Required:


Plasma: 1.0 mL

Collection Information: 

·        The recommended collection time is 7-10 a.m., after the patient is active (walking, standing, seating) for at least 2 hours. Patient must be seated for 5-15 min prior to collection.

·        Collect EDTA plasma. Serum or lithium heparin plasma are not acceptable.

·        Do not pre-chill EDTA blood collection tubes nor store tubes on ice, but process blood at room temperature to avoid cryoactivation of prorenin in renin testing.

·        Centrifuge tubes in a non-refrigerated centrifuge, separate EDTA-plasma from cells immediately after centrifugation, then aliquot and deep-freeze at -20 °C or below instantly.

Specimen Stability:

Ambient: 6 hours

Refrigerated: not stable

Frozen: 3 days at -20oC or at least 1 month at -80°C

 

Referral:
Plasma: 1.0 mL
Pediatric Plasma: 0.8 mL

Shipping & Storage: Store and ship frozen. Preferably on dry ice if available.

Testing Laboratory (MB): Send specimen to Health Sciences Centre - MS5

 

Requisition:
Reference Values:
Reference Intervals:
Age

Gender

Reference interval
0 -<3y

M/F

7.7-172 mIU/L
3 - <18y

M/F

9.4-93 mIU/L
≥18y

M/F

Upright: 4.4 - 46 mIU/L 

Supine:  2.8 - 40 mIU/L   


Method of Analysis: DiaSorin Chemiluminescence

 

 

Availability:
Within 1 Week
See Also:
More Information:
Interpretation & Assay Interferences: The assay is not affected by concentrations of bilirubin up to 40 mg/dL, hemoglobin up to 600 mg/dL, or triglycerides up to 3000 mg/dL.


References: Ömer Özcan, Jacquelien J Hillebrand, Wendy den Elzen, Annemieke C Heijboer, The Clinical Impact of Sample Storage at -20 °C on Renin Reference Intervals and Aldosterone–Renin Ratio Calculations, The Journal of Clinical Endocrinology & Metabolism, 2024;, dgae057, https://doi.org/10.1210/clinem/dgae057