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Laboratory: Clinical Immunology
Test Name:
Test Code: IBD
Clinical Information:
IBD Testing includes ANCA and ASCA (IgG/IgA).
Testing is performed at St. Boniface Hospital Immunology Lab using a combination of indirect Immunofluorescence assay (IFA) and enzyme-linked immunosorbent assay (ELISA). 
Cytoplasmic Neutrophilic Antibodies (ANCA) is designed for the determination of human antibodies of immunoglobulin class IgG against granulocyte cytoplasm in patient samples for the diagnosis of chronic inflammatory bowel diseases, mainly ulcerative colitis.   Serum samples are initially screened at a 1:10 dilution.
Anti-Saccharomyces cerevisiae IgG/IgA (ASCA) is a quantitative in vitro assay for human antibodies of the IgG & IgA class against Saccharomyces cerevisiae in serum or plasma for patients suspected of Crohn’s Disease.
Collection Devices:
Specimen Required:
Blood: 5.0 mL

Serum: 1.0 mL
Serum: 1.0 mL

Separate serum and ship refrigerated, or frozen if greater than 7 days from time of collection.
Reference Values:
ANCA:           <1:10 Titre (Negative)
ASCA IgG:    0 – 19 RU/ml
ASCA IgA:    0 – 19 RU/ml
A negative result does not necessarily exclude autoimmune disease.  Positive results should not be interpreted in isolation and must be correlated with relevant clinical findings.
Laboratory Hours of Operation: Mon-Fri, 0800-1615, excluding holidays. ANCA TAT: Within 4 Days; ASCA TAT: Within 10 Days
See Also:
More Information:
c-ANCA and p-ANCA are reported as Negative, Positive, Atypical or Indeterminate.  All Positive c-ANCA results will automatically reflex PR3 testing while Positive p-ANCA results will reflex MPO testing.  Indeterminate ANCA results will reflex both MPO and PR3 testing.
Positive ANCA results will also be accompanied by a Titre.
ASCA reportable range: 2-200 RU/ml