| Laboratory: | Clinical Immunology | ||||||||||||||||||||||||||||||
| Test Name: |
ANTI-NUCLEAR ANTIBODIES - (S)
Test Code:
HEP2
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| Clinical Information: |
Alternate Names:
ANA ANA IFA ANA Screen Antinuclear Antibody HEP-2 Fluorescent Antinuclear Antibodies Evaluation of patients suspected of having systemic autoimmune rheumatic disease (ANA-associated rheumatic diseases or connective tissue disease) or organ-specific autoimmune diseases such as autoimmune liver diseases. DO NOT order dsDNA as a routine screen with ANA. Visit the International Consensus on Antinuclear Antibody Patterns website for additional information about pattern and disease/antigen associations. |
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| Collection Devices: |
Preferred Device:
1 - Serum Separator (SST) Tube(s) - Full Tube Collection
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| Specimen Required: |
Serum: 1.0 mL
Refrigerated: 14 days Frozen: 30 days |
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| Referral: |
Serum: 1.0 mL
Separate serum and ship refrigerated, or frozen if greater than 7 days from time of collection.
Testing Laboratory: St. Boniface Hospital Immunology Laboratory |
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| Requisition: | |||||||||||||||||||||||||||||||
| Reference Values: |
Reference Intervals:
Negative <1:80 Titre A negative result does not necessarily exclude autoimmune disease. Positive results should not be interpreted in isolation and must be correlated with relevant clinical findings. Note: 10% of normal individuals will have a positive ANA at 1:80 titre. Method of Analysis: The indirect immunofluorescence test (IFA) is the gold standard for the determination of antibodies against nuclear antigens (ANA), including cytoplasmic components. HEP-20-10 cell substrate is utilized (HEP-2 cells optimised to form more mitotic stage cells). Serum samples are initially screened at a 1:80 dilution. EUROIMMUN Hep-20-10 EUROPattern Instructions for the indirect immunofluorescence test, FC_1522_A_UK_C16.doc Version 11/01/2017. |
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| Availability: |
Weekdays
TAT: Within 4 days
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| See Also: | |||||||||||||||||||||||||||||||
| More Information: |
Positive results will be accompanied by a pattern (Nuclear,Cytoplasmic, Mitotic) and titre.
Reflex testing will be performed on titres ≥ 160 as per the table below:
ANA Profile will be performed by Line Immunoassay and will include nRNP/Sm, Sm, SS-A, Ro-52, SS-B, Scl-70, PM-Scl, Jo-1, CENP B, PCNA, Nucleosomes, Histones, Rib P Protein, AMA-M2 and DFS70. |
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