Laboratory: Clinical Immunology
Test Name:
Test Code: ANCA
Clinical Information:
Testing is performed at St. Boniface Hospital Immunology Lab using an indirect Immunofluorescence assay (IFA).  Serum samples are initially screened at a 1:10 dilution.
For Vasculitis testing, ANCA is orderable as a stand-alone test.
For Inflammatory Bowel Disease, ANCA is included in a panel with ASCA IgG/IgA (Testcode IBD)

This test is designed for the determination of human antibodies of immunoglobulin class IgG against granulocyte cytoplasm (ANCA) in patient samples for the diagnosis of ANCA-associated vasculitis, for example Wegener’s granulomatosis, microscopic polyangiitis, pauciimmune necrotizing glomerulonephritis, polyarteritis nodosa, Churg-Strauss syndrome and primary sclerosing cholangitis and chronic inflammatory bowel diseases, mainly ulcerative colitis.
Approximately 90% of patients with a P-ANCA pattern by IFA have antibodies specific for myeloperoxidase (MPO). Approximately 85% of patients with a C-ANCA pattern by IFA have antibodies specific for serine proteinase 3 (PR-3).
Collection Devices:
Specimen Required:
Blood: 5.0 mL

Serum: 1.0 mL
Serum: 1.0 mL

Separate serum and ship refrigerated, or frozen if greater than 7 days from time of collection.
Reference Values:
<1:10 Titre (Negative)
A negative result does not necessarily exclude autoimmune disease.  Positive results should not be interpreted in isolation and must be correlated with relevant clinical findings.
Laboratory Hours of Operation: Mon-Fri, 0800-1615, excluding holidays. ANCA TAT: within 4 days
See Also:
More Information:
c-ANCA and p-ANCA are reported as Negative, Positive, Atypical or Indeterminate.  All Positive c-ANCA results will automatically reflex PR3 testing while Positive p-ANCA results will reflex MPO testing.
Indeterminate ANCA results will reflex both MPO and PR3 testing.
Positive results will also be accompanied by a Titre.