Lab Information Manual

Laboratory:

Clinical Biochemistry (SENDOUTS)

Test Name:

VITAMIN D (1,25-DIHYDROXY) - (S)

Test Code: D125

Clinical Information:

Alternate Name(s): Calcitriol, 1,25-Hydroxy Vitamin D; 1,25-Dihydroxyvitamin D
Test Indications: This test is not appropriate for diagnosing vitamin D deficiency or insufficiency. For this purpose, see: VITAMIN D (25-HYDROXY) - (S)

1,25-OH vitamin D should only be ordered in the following clinical conditions:

1) Clinical evidence of vitamin D deficiency due to hereditary deficiency of renal 1-alpha hydroxylase or end-organ resistance to 1,25-dihydroxy vitamin D.
2) Hypercalcemia or renal failure in addition to Vitamin D, 25-Hydroxy testing.

Test Approval Requirements: F260-11-02 Biochemistry Approval for Test Referral Form

This test is available without approval to Endocrinologist and Nephrologist only.

All other requests must be approved by a clinical biochemist using the designated test approval form.

Clinical Practice Change - Endocrine Testing and Specialized Endocrinology Requisition Update 15-May-24

Clinical Practice Change General-Endocrinology-Requisition-and-Endocrine-Testing-Update 15-May-24

Collection Devices:

Preferred Device:	1 - Serum Tube(s), NO GEL - Full Tube Collection Serum Tube(s), NO GEL - Full Tube Collection Manufacturer: Becton Dickinson Catalogue #: 366430
Alternate Device:	1 - Microtainer(s) - Plain Serum, NO GEL (0.5 mL) to fill line

Serum GEL tubes and Barricor^TM tubes are not acceptable.

Specimen Required:

Serum: 2.0 mL

Pediatric Serum: 1.0 mL

Specimen Stability:

Ambient: 2 days

Refrigerated: 14 days

Frozen: >1 year

Referral:

Shipping & Storage: Separate and freeze as soon as possible. Store and send frozen.

Referred Out Location:

Specimen referred from HSC-MS5 to:
In-Common Laboratories
57 Gervais Drive
North York, Ontario
M3C 1Z2
Telephone: (416) 422-3000
Test ID: 125VITD
Testing location: Mount Sinai Hospital, 600 University Avenue, Toronto ON

Requisition:

_SH General Endocrinology Requisition (R250-10-76)

_SH Specialized Endocrinology Requisition (R250-10-77)

Reference Values:

Age	Reference Interval
< 1 year	77 - 471 pmol/L
1-2 y	113 - 363 pmol/L
3-18 y	108 - 246 pmol/L
>=19 y	48 - 190 pmol/L

Method of Analysis: DiaSorin Chemiluminescence

Availability:

Within 2 Weeks

See F10-50-45D Approved Physicians for Specialist Endocrinology Requisition